一炕四女被窝交换全文阅读,老熟妇仑乱一区二区视頻,无码国内精品人妻少妇蜜桃视频,浴室高潮bd在线观看

歡迎進入蘇州阿爾法生物實驗器材有限公司!
技術(shù)文章
首頁 > 技術(shù)文章 > 瓊脂糖預(yù)染預(yù)制膠說明書

瓊脂糖預(yù)染預(yù)制膠說明書

 更新時間:2023-12-13 點擊量:898

*運輸及保存

*Shipping and Storage
瓊脂糖預(yù)染預(yù)制膠電泳試劑盒的小黑盒 2~8℃保存和運輸,有效期 12 個月。
Ship and store the kit at 2~8℃. It will remain stable for one year.
專適 6×DNA Loading Buffer 2~8℃運輸,長期需要-20℃保存,有效期 12 個月。
Ship Optimized 6×DNA loading buffer at 2~8℃,for short-time storage and at -20℃ for long-time storage. It will remain stable for one year.
TAE 速溶顆粒 2~8℃或常溫運輸,常溫保存,有效期 24 個月。
Ship TAE Instant Granule at 2~8℃ or room temperature and store at room temperature. It will remain stable for two years.
專適 Marker 2~8℃運輸,長期需要-20℃保存,有效期 12 個月。
Ship Optimized Marker at 2~8℃ and store it at -20°C for long-time storage. It will remain stable for one year.
*自備試劑
*Reagents Required But Not Provided
核酸樣品、去離子水
Nucleic acid sample and Deionized water
*使用方法
*Procedure
1.量取約 600ml 的蒸餾水加入燒杯,并放置一個磁性攪拌子于燒杯中。將燒杯置于磁力攪拌器上,慢慢加入 1 袋 TAE 速溶顆粒的全部內(nèi)容物,攪拌溶液直至
溶解。把燒杯中的溶液倒入 1000ml 的容量瓶中,再加入蒸餾水,定容至 1000ml,即為 1×TAE 溶液。
1. Add one pouch of TAE Instant Granule into the cleaned beaker, dissolved completely with 600 ml distilled water under a magnetic stirrer. Pour the solution into 1L flask. Add distilled water to the
solution until the total volume is 1L. The final solution is 1×TAE buffer.
2.取出一塊獨立包裝的瓊脂糖預(yù)染預(yù)制膠,撕掉表面的塑料膜,反轉(zhuǎn)包裝,用兩手的食指和中指托住塑料殼邊緣,開口向下沒入電泳液中,然后用兩個大拇指輕
輕按壓塑料殼背面中心部分,瓊脂糖預(yù)染預(yù)制膠就會落入電泳液中,此時的預(yù)制膠帶孔面向上,移動膠塊,使孔側(cè)端靠近電泳槽負極。如樣品孔內(nèi)有氣泡,應(yīng)設(shè)法除
去。
2. Take out one kit, take off the plastic package, reverse it, support the two edges with index and middle fingers of both hands, immerse it in the buffer with the opening downward and gently press the
central part of the kit with two thumbs. Thus the gel will fall into the buffer with the side of the well upward. Move the gel to make the well end close to negative electrode of the electrophoresis cell. If
bubbles are produced in the sample wells, try to remove them.
3.按 5:1 的比例取適量核酸樣品和專適 6×DNA Loading Buffer 混勻,用移液器將專適 Marker 和樣品混合液依次緩慢加入被浸沒的凝膠加樣孔內(nèi)。
3. Mix Optimized 6×DNA loading buffer and DNA sample at a volume ratio of 1:5. Carefully load prepared Marker and the mixed sample into the wells with pipette successively.
4.接通電源,紅色為正極,黑色為負極,切記 DNA 樣品由負極往正極泳動(靠近加樣孔的一端為負)。
4. Connect the electrophoresis cell to the power source according to the conventions: Red-Anode and Black-Cathode. Turn on the power source. Note that the DNA sample moves from the negative to
the positive (the end near the wells that DNA samples are loaded in is negative).
5.根據(jù)指示劑泳動的位置,判斷是否終止電泳。
5. Determine whether to stop electrophoresis according to the migration of the tracking dyes.
6.電泳完畢,關(guān)閉電源,用凝膠成像儀觀察電泳條帶及其位置,與 Marker 比較擴增產(chǎn)物的大小。
6. Switch off the power source when the electrophoresis finishes. Visualize the band by using a gel documentation system and compare the size of the amplified product with that of Marker


預(yù)制膠取較操作方法




蛋白預(yù)制膠取膠方法


一个人看的www免费视频| 99久久人妻无码精品系列蜜桃| 精品国产人妻一区二区三区| 亚洲欧美人成无码苍井空| 按摩师舌头进去添的我好舒服| 天下第一日本www视频| 破外女13一14在线观看| 试看a片成人免费区| 国产成人一区二区三区影院动漫| 99久久伊人精品综合观看| 99热这里有精品| 免费看片a级毛片免费看 | 狠狠色噜噜狠狠狠狠AV不卡| 国产一区二区三区播放心情潘金莲| 女人18毛片水真多| 无码人妻久久一区二区三区不卡 | 欧美成人在线视频| 午夜男女爽爽影院a片免费| h女主从小被c到大1v1| 国产精品久久久久久亚洲毛片| 欧洲VODAWIFI喷浆3D| 理科生坠入情网电视剧免费观看| 人妻久久久一区二区三区| 欧美激情性做爰免费视频| 不说就继续这样弄你| 高清无码在线观看| 亚洲va欧美va天堂v国产综合| 日韩精品无码一区二区中文字幕| 玩弄人妻少妇500系列视频| 人妻办公室被强奷hd| 全国最大成人网站| 欧美乱大交XXXXX潮喷| 亚洲精品乱码久久久久久蜜桃图片 | 无码av一区二区三区| 国产精品久久久久久久久免费蜜桃 | 亚洲av片不卡无码久久蜜芽| 亚洲av成人精品网站在线播放| 激烈 痉挛 抽搐 潮喷 MP4| gogo全球专业高清摄影| 色综合天天综合网国产成人网| 亚洲成av人在线视|